Peroxynitrite Scavenging and Singlet Oxygen Scavenging Antioxidant, Antifungal Activity and Investigation of Natural Metabolites of Pineapple (Ananas comosus) Peels Ethanolic Extract Using GC-MS Technique
Keywords:
Peroxynitrite, Singlet Oxygen Scavenging, Antifungal, Metabolites of Pineapple, Peels, GC-MS TechniqueAbstract
The pineapple has been extensively cultivated due to the high value of the product in the market and high production volume. Harvest has a crucial part played by the ripening stage that affects sensory quality and shelf life, particularly the non-climacteric fruit since they do not ripen after picking. The aim of this investigation was to study antioxidant and antifungal activity of pineapple metabolites based on metabolite profiling method. The results of the small molecule study revealed thirteen compounds which include alpha-Farnesene, (3Z,6Z), Palmitic acid-1,2-13C2, Tricin, Feruloylputrescine, n-Decanal, 2,4-dichlorobenzoic acid, Caryophyllene, 1-o-p-coumaroylglycerol, Chrysoeriol-7-O-glucoside, alpha-Myrcene, N,-p-Coumaroyl-N,fer Antioxidants chemical substances have the capability to prevent degenerative diseases by supplying electrons or electrons to free radicals. Fruit peroxide scavenging Fruit chemical such as crude methanolic extract of the uproot fructus; ethyl acetate, ethanol, and gallic acid (standard) expressed were 709.00±25.45, 645.80±20.00, 673.31±22.10 and 812.00±29.07 while recorded 49.00±3.71, 28.50±2.46, 36.09±2.00 and 43.26±2.19 respectively for Singlet oxygen scavenging. When compared to amphotericin B (Am B) and nystatin (NY), the antifungal activity of methanol and ethanol, which are natural metabolites of pineapple (Ananas comosus) peels, were 25.91±0.35, 21.05±0.29, 35.00±0.58 and 31.64±0.53 in Candida albicans while recorded 15.40 ±0.24, 17.09±0.25, 24.09±0.33 and 22.00±0.31 in Cladosporium herbarum. In the same time recorded 16.70±0.25, 11.98±0.21, 17.00±0.25 and 22.04±0.29 for Trichophyton rubrum. Antifungal activity of Fusarium oxyporum 15.12±0.24, 20.09±0.27, 21.09±0.28 and 27.00±0.41 while record 29.00±0.43, 23.08±0.32, 37.90±0.59 and 29.04±0.54 in Cladosporium herbarum.
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