Proteus mirabilis Profile Metabolites Isolated in Patients with Urinary Tract Infection and Its Antibacterial Activity
Keywords:
Proteus mirabilis, patients, UTI, Antibacterial activityAbstract
Examining the bioactive chemical compounds and their antibacterial activity were the goals of this research. The bioactive chemicals or secondary metabolites of the methanolic extract were analyzed using (GC-MS) techniques after which its biomedical properties of antibacterial activity in vitro were tested. Proteus mirabilis was analyzed by GC-MS, showing the presence of the: 1-(4-cycloprolphenyl)-ethenone, 1,3-benz-dioxole, 2-Methoxy-4-(propyl)phenol, Apiol, Pyrazolo-5-a]pyridine, 2-Methyl-phenyl-2-propanol, 2,3-Dimethoxy-5-(1-propenyl)phenol, 2-Propenamide, 2-methyl, 1-benzylindoline-2,3-dione, 4-Oxocyclohexanecarboxylic acid, 4-Amino-3,5-dinitrobenzotrifluoride, 2,5-Dimethyltetrahydrofuran-3-thiol, Hexadecanoic acid, 2-methoxyethyl ester, Isophthalaldhyde, 5-Ethoxy-4-Hydroxy, d-Methylamphetamine and 1,1,3,3-Tetramethoxypropane. The antimicrobial activity of secondary metabolites of Proteus mirabilis which is an isolate of a urinary tract infection was tested against six different pathogenic microorganisms. The results were compared to those of two conventional antibiotics, AP-Ampicillin and RF-Rifampicin. Antibacterial activity of bioactive secondary metabolites was (14.09±0.25, 23.07±0.34, and 16.45 ±0.29), (10.30±0.21, 17.00±0.31, and 18.96±0.34), (11.00±0.20, 23.89±0.35, and 17.45±0.30), (08.05±0.17, 19.00±0.36, and 12.01±0.24), (13.00±0.20, 23.05±0.33, and 27.81±0.40), and (15.04±0.28, 24.90±0.37, and 21.85±0.32) against Streptococcus pyogenes, Enterococcus faecalis, Bacillus cereus, Bacillus subtilis, Staphylococcus epidermidis and Staphylococcus aureus respectively. The demonstrated results of the anti-bacterial activity indicated that the volatile chemicals produced by the Proteus mirabilis had a positive effect on the growth of Staphylococcus aureus (15.04±0.28).
Downloads
References
Matsuyama T, Takagi Y, Nakagawa Y, Itoh H, Wakita J, Matsushita M. 2000. "Dynamic aspects of the structured cell population in a swarming colony of Proteus mirabilis". J. Bacteriol.182 (2): 385–93.
Kokila, V., Namasivayam, S.K.R. and Amutha, K. 2024. Hypocholesterolemic potential of Bacillus amyloliquefaciens KAVK1 modulates lipid accumulation on 3T3-L1 adipose cells and high fat diet-induced obese rat model. World J. Microbiol. Biotechnol. 40, 206.
Linde, G.A., Gazim, Z.C., Cardoso, B.K., Jorge, L.F., Tešević, V., Glamočlija, J., Soković, M. and Colauto, N.B. 2016. Antifungal and antibacterial activities of Petroselinum crispum essential oil. Genet. Mol. Res. 15(3), 15038538.
Mahboubi, M., Mahdizadeh, E. and Tabar, R.H. 2019. Chemical composition and antimicrobial activity of Fraxinus excelsior L. seeds essential oil. Jundishapur J. Nat. Pharm. Prod. 14(1):e61105
Marston, A., Hostettmann, K. and Msonthi, J.O. 1995. Isolation of antifungal and larvicidal constituents of Diplolophium buchanani by centrifugal partition chromatography. J. Nat. Prod. 58, 128–130. Mohammed, H.A. and Zgair, A.K. 2022. Detection of quorum sensing genes of Pseudomonas aeruginosa isolated from different areas in Iraq. Iraqi J. Sci. 63(11), 4665–4673.
Mohanty, S., Baliyarsingh, B. and Nayak, S.K. 2021. Antimicrobial resistance in Pseudomonas aeruginosa: a concise. Antimicrob. Resist. One Health Perspect. 49, 177–192.
Moussaoui, O., Bhadane, R., Sghyar, R., El Hadrami, E.M., El Amrani, S., Ben Tama, A., Rodi, Y.K., Chakroune, S. and Salo-Ahen, O.M.H. 2020. Novel amino acid derivatives of quinolines as potential antibacterial and fluorophore agents. Sci. Pharm. 88, 57.
Munita, J.M. and Arias, C.A. 2016. Mechanisms of antibiotic resistance. Microbiol. Spectr. 4(2), 481 511.
Liaw, S.J.; Lai, H.C. and Wang, W.B. 2004. Modulation of swarming and virulence by fatty acids trough the RsbA protein in Proteus mirabilis. Infect. Immun.72: 6836–6845.
AL-Kaebi S.J. and Matrood, A. J. 2011. The effect of urea and ethanol on swarming phenomenon of local isolate of Proteus mirabilis. J. ThiQar. UN.6(4):41.
Senior, B. W., L. M. Loomes, and M. A. Kerr. 1991. The production and activity in vivo of Proteus mirabilis IgA protease in infections of the urinary tract. J. Med. Microbiol. 35:203–207
Nagoor, M.F. and Prince, S.M.P. 2012. Protective effects of thymol on altered plasma lipid peroxidation and nonenzymic antioxidants in isoproterenol-induced myocardial infarcted rats. J. Biochem. Mol. Toxicol. 26(9), 368–373.
Namasivayam, S.K.R., Jerry Angel, J., Arvind Bharani, R.S. and Valli Nachiyar, C. 2020. Terminalia chebula and Ficus racemosa principles mediated repression of novel drug target Las R—the transcriptional regulator and its controlled virulence factors produced by multiple drug resistant Pseudomonas aeruginosa—biocompatible formulation against drug resistant bacteria. Microb. Pathog. 148, 104412.
Pham, T.D., Ziora, Z.M. and Blaskovich, M.A. 2019. Quinolone antibiotics. Medchemcomm, 10, 1719 1739.
Qinghua, S., Yangmin, M. and Huqiang, Q. 2005. Preliminary study on chemical composition and insecticidal activity of root bark from Periploca sepium. Acta Agric. Bor. Sin. 14(6), 141–144.
Ramaroson, M.L., Koutouan, C., Helesbeux, J.J., Le Clerc, V., Hamama, L., Geoffriau, E. and Briard, M. 2022. Role of phenylpropanoids and flavonoids in plant resistance to pests and diseases. Mol. 27, 8371.
Razack, S., Hemanth Kumar, K., Nallamuthu, I., Naika, M.and Khanum, F. 2015. Antioxidant, biomolecule oxidation protective activities of Nardostachys jatamansi DC and its phytochemical analysis by RP-HPLC and GC-MS. Antioxid. 4(1), 185–203.
Razook, B.R.F., Al-ani, A.N. and Mahmood, M.M. 2020. Hematological picture of rabbits immunized with Pseudomonas aeruginosa. Iraqi J. Vet. Med. 44(E0), 64–68.
Rossi, E., La Rosa, R., Bartell, J.A., Marvig, R.L., Haagensen, J.A.J., Sommer, L.M., Molin, S. and Johansen, H.K. 2021. Pseudomonas aeruginosa adaptation and evolution in patients with cystic fibrosis. Nat. Rev. Microbiol. 19(5), 331–342.
Schmittgen, T.D. and Livak, K.J. 2008. Analyzing real time PCR data by the comparative CT method. Nat. Protoc. 3(6), 1101–1108.
Sethupathy, S., Prasath, K.G., Ananthi, S., Mahalingam, S., Balan, S.Y. and Pandian, S.K. 2016. Proteomic analysis reveals modulation of iron homeostasis and oxidative stress response in Pseudomonas aeruginosa PAO1 by curcumin inhibiting quorum sensing regulated virulence factors and biofilm production. J. Proteom. 145, 112–126.
Fujihara, M., Obara, H., Watanabe, Y., Ono, H.K., Saski J., Goryo, M., Harasawa, R. 2011. Acidic environments induce differentiation of Proteus mirabilis into swarmer morphotypes. Microbiol. Immunol.55: 489–493.
Jansen, A. M.; V. Lockatell, D. E. Johnson, H. L. Mobley. 2004. Mannose-resistant Proteus-like fimbriae are produced by most Proteus mirabilis strains infecting the urinary tract, dictate the in vivo localization of bacteria, and contribute to biofilm formation. Infect. Immun. 72:7294–7305.
Hay NA, Tipper D, Gygi, D, Hughes, C. A nonswarming mutant of Proteus mirabilis lacks the Lrp global transcriptional regulator. J. Bacteriol. 1997; 179: 4741–4746
Shah, S.M. and Abdul-Jalil, T.Z. 2022. Qualitative and quantitative estimation or chemical constituents from leaves and roots of Iraqi Agave Attenuata by GC-MS and RP-HPLC (Conference Paper). Iraqi J. Pharm. Sci. 31(Suppl.), 75–85.
Sowmya, R. and Namasivayam S.K.R. 2024. Pesticidal activity of extra cellular metabolites extracted from the green muscardian fungi Metarhizium anisopliae. Environ. Qual. Manag. 34(1), e22235.
Srivastava, S., Gupta, M.M., Prajapati, V., Tripathi, A.K. and Sushil Kumar. 2001. Insecticidal activity of Myristicin from Piper mullesua. Pharm. Biol. 39(3) 226–229.
Tisserand, R. and Balacs, T. 1994. Nutmeg: A review of the toxicology and pharmacology of nutmeg and its essential oil. Int. J. Aromather. 6, 28–38.
Uma, B., Prabhakar, K., Rajendran, S. and Lakshmi Sarayu, Y. 2009. Studies on GC/MS spectroscopic analysis of some bioactive antimicrobial compounds from Cinnamomum zeylanicum. J. Med. Plants. 8(31), 125–131.
Pearson MM, Mobley HL. 2008. Repression motility during fimbrial expression; identification of 14 mrpJ gene paralogues in Proteus mirabilis. Mol. Microbiol. 69: 548–558
Morgenstein, RM, Szostek, B, Rather PN. 2010. Regulation of gene expression during swarmer cell differentiation in Proteus mirabilis. FEMS Microbiol. Rev. 34: 753–763.
Belas R, Manos J. Suvanasuthi, R. 2004. Proteus mirabilis ZapA metalloprotease degrades a broad spectrum of substrates, including antimicrobial peptides. Infect Immun. 72(9): 5159-67.
Downloads
Published
How to Cite
Issue
Section
License
This work is licensed under a Creative Commons Attribution 4.0 International License.
Current Clinical and Medical Education
